anti apoa i  (R&D Systems)

Journal: bioRxiv

Article Title: Combining Anion Exchange and Size Exclusion Chromatography for Extracellular Vesicle Enrichment from Small Volumes of Human and Mouse Plasma for Quantitative Proteomics

doi: 10.64898/2026.03.11.711200

Figure Lengend Snippet: Workflow for EV enrichment from 4 ml (A) and 0.5 ml (B). EV markers (CD63 and Syntenin-1) and plasma contaminant proteins (Albumin and ApoA1(shown in red)) by western blot analysis (panel C). Starting plasma volume, final volume post concentration with the centrifugal filter and gel loading volume were used to calculate the concentration factor and equivalent plasma starting amounts indicated above figure.

Article Snippet: Membranes were blocked in 5% skim milk in PBS at room temperature for 1 hour before overnight incubation with primary antibodies Syntenin (Abcam, cat# ab133267), CD63 (Abcam, cat#ab134045), CD63 (Abcam, cat#ab8219), Albumin (Cell Signalling Technology, cat#4929S), Calnexin (Cell Signalling Technology, cat#2679S) ApoA1 (Santa Cruz, cat# SC-376818).

Techniques: Clinical Proteomics, Western Blot, Concentration Assay

Journal: bioRxiv

Article Title: Combining Anion Exchange and Size Exclusion Chromatography for Extracellular Vesicle Enrichment from Small Volumes of Human and Mouse Plasma for Quantitative Proteomics

doi: 10.64898/2026.03.11.711200

Figure Lengend Snippet: SEC Void optimisation. Pooled SEC fractions 1-4 collected using a 2.7 mL void volume (1-4A) and a 2.8 mL void volume (1-4B) were prepared in triplicate from 0.5 mL plasma. Western blot analysis of contaminant proteins Albumin (green) and lipoprotein ApoA1 (red) (A) with 0.5 µl plasma included as a positive control (+). EV markers CD63 and syntenin-1 and small EV exclusion marker Calnexin (B). Samples (0.5 ml and 0.4 ml) from Experiment 3.2 included as a western blot control and 2µl platelet lysate included as a calnexin positive control (+). Electron microscopy (C) visualisation and size and concentration histogram (D) of EV representative SEC sample (Void 2.7 ml).

Article Snippet: Membranes were blocked in 5% skim milk in PBS at room temperature for 1 hour before overnight incubation with primary antibodies Syntenin (Abcam, cat# ab133267), CD63 (Abcam, cat#ab134045), CD63 (Abcam, cat#ab8219), Albumin (Cell Signalling Technology, cat#4929S), Calnexin (Cell Signalling Technology, cat#2679S) ApoA1 (Santa Cruz, cat# SC-376818).

Techniques: Clinical Proteomics, Western Blot, Positive Control, Marker, Control, Electron Microscopy, Concentration Assay

Journal: Neurochemical Research

Article Title: Plasma-Derived Exosomal hsa-miR-3677-3p Induces Ferroptosis in Neurons by Targeting ABCB8 in Perioperative Neurocognitive Disorders After Prostate Surgery

doi: 10.1007/s11064-026-04665-2

Figure Lengend Snippet: Characterization of exosomes isolated from plasma in patients with dNCR or Non-dNCR after prostate surgery. A Representative Western blot analysis of exosomal markers (HSP60, TSG101, CD9 and CD81). The negative markers for exosomes, including APOA1, Calnexin, and GAPDH, were assessed in the protein lysates of exosomes, with human plasma supernatant and SH-SY5Y cell lysate samples serving as positive controls ( n = 5/group). B Transmission electron microscopy (TEM) images of plasma-derived exosomes from non-dNCR and dNCR patients. C Exosome samples were analyzed using Nanoparticle Tracking Analysis (NTA). The calculated size distribution is illustrated with the mean (black line) and the standard error (red shaded area). The mean, mode, and median particle sizes are presented along with their standard deviations (± SD). D NTA analysis of plasma-derived exosomes from non-dNCR and dNCR patients, showing size distribution (median diamete, nm) and particle concentration (particles/mL) ( n = 5/group). Statistical test (D): Student’s t test. Error bars denote SEM. ns: not significant

Article Snippet: Immunoblots were probed with purified primary antibodies for HSP60 (ab190828, rabbit, 1:1000, Abcam, USA), TSG101 (ab133586, rabbit, 1:2000, Abcam, USA), CD81 (ab155760, rabbit, 1:1000, Abcam, USA), CD9 (PC22293, rabbit, 1:1000, Abmart, China), ALIX ( T57215 , rabbit, 1:1000, Abmart, China), APOA1 (DF6264, rabbit, 1:500, Affinity Biosciences, China), Calnexin (ab92573, rabbit, 1:1000, Abcam, USA) or GAPDH (33033, mouse, 1:2000, Bioss, China) overnight at 4 °C, followed by incubation with horseradish peroxidase (HRP)-conjugated goat anti-mouse (A4416, 1:10,000) or goat anti-rabbit (A0545, 1:10,000) secondary antibodies (Cell Signaling Technologies, USA).

Techniques: Isolation, Clinical Proteomics, Western Blot, Transmission Assay, Electron Microscopy, Derivative Assay, Concentration Assay